丙烯酰胺诱导小鼠淋巴瘤细胞tk基因突变的研究

doi:10.3969/j.issn.1004-616x.2005.04.003

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (4): 202-205.doi: 10.3969/j.issn.1004-616x.2005.04.003

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Acrylamide Induce tk Gene Mutation in Mouse Lymphoma Cells

YUAN Jian;LIU Sheng-xue; CAO Jia;XIONG Yan

Department of Hygiene Toxicology, College of military Preventive Medicine, Third Military Medical University, Chongqing 400038,China

Received:2004-07-21 Revised:2004-12-13 Online:2005-07-30 Published:2005-07-30
Contact: CAO Jia

Abstract

Abstract: BACKGROUND ＆ AIM: To investigate the effect of acrylamide(AA) on the tk gene of mouse lymphoma cells. MATERTAL AND METHODS: L5178Y cells were treated with AA at different concentrations. Determination of cytotoxicity, cell inoculation efficiency, relative survival rate and mutation frequency was performed. RESULTS: The relative survival rate and suspension growth rate of L5178Y cells decreased significantly with the increasing doses of AA. The mutation frequency of tk gene induced by AA(150～750 μg/ml) was 1～9 times higher than that of spontaneous mutation frequency of L5178Y cells. CONCLUSION: AA have obvious cytotoxic and mutagenic effects on tk gene in L5178Y cell.

Key words: acrylamide, tk locus, mutagenesis, L5178Y cell

YUAN Jian, LIU Sheng-xue, CAO Jia, XIONG Yan. Acrylamide Induce tk Gene Mutation in Mouse Lymphoma Cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(4): 202-205.

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Acrylamide Induce tk Gene Mutation in Mouse Lymphoma Cells

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